Figure 7.

Lactobacillus acidophilus (LA) increases phosphorylation of ezrin and stimulates Na⁺/H⁺ exchanger 3 (NHE3)-NHE regulatory factor 2 (NHERF2) interaction. A: protein expression was determined in Caco-2bbe cells treated with or without LA conditioned media (LCM) for 6 h. Representative blots of three independent experiments are shown. B: surface membrane abundance of NHE3 in Caco-2bbe cells overexpressing protein kinase C (PKC)α was determined. Relative surface to total NHE3 ratios are shown in the lower panel. Data (means ± SE) are combined from four independent experiments using cells of different passages. C: quantification of NHERF2 expression (left) and ezrin phosphorylation (right) in control transfected (clear bars) and PKCα overexpressing cells (shaded bars). Data are combined from two independent experiments. D: expression levels of phospho-ezrin and total ezrin were determined. E: NHE3 was immunoprecipitated from biotinylated membrane pools and coimmunoprecipitated NHERF2 was determined. Clear bars, controls; shade bars, PKCα overexpressing cells. NHERF2 to NHE3 ratios are shown below. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.