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Confocal immunofluorescence images of Caco-2 (left = static; right = perfused) at 14 days from the seeding. (a) F-actin was stained in green, DNA in blue, and mucin-2 in red. (b) F-actin was stained in green, DNA in blue, and occludin in red. (c) the DNA was stained in blue and zonulin-1 in red. The first row corresponds to the apical plane (AP) of the villus, and the row below, to the basal plane (BP). The scale bar corresponds to 25 μm.
