Table 6.
Effect of T. erecta leaves fractions (ethyl acetate, butanol) & acacetin 7-O-glucoside on each of the following: Bax and Bcl2 gene expression
| RT-PCR (gene expression) | Group 1 Control | Group 2 Den [55 mg/kg] | Group 3 Den + DoX [10 mg/kg] | Group 4 Den + EB [50 mg/kg] | Group 5 Den + EE [50 mg/kg] | Group 6 Acacetin 7-O-glucoside [0.44 mg/ml] |
|---|---|---|---|---|---|---|
| Bax | 0.003 ± 1 | 3.6 ± 0.08* | 2.9 ± 0.08*# | 1.86 ± 0.05*# | 1.83 ± 0.06* | 1.4 ± 0.03 |
| Bcl2 | 1 ± 0.005 | 0.2 ± 0.05* | 0.7 ± 0.03*# | 1.8 ± 0.05# | 1.56 ± 0.03# | 2.3 ± 0.17 |
| Bax/Bcl2 | 1 ± 0.006 | 14 ± 2.1* | 3.8 ± 0.25# | 0.99 ± 0.003*# | 1.106 ± 0.02*# | 0.6 ± 0.025 |
Data are represented as mean ± SEM (n = 6). *p <0.05 vs. normal control, #p <0.05 vs Den, *#p <0.05 vs Den + Dox. Statistical analysis was done using one-way ANOVA followed by the Dunnett test as multiple comparison test. ALT, analine aminotransferase; AST, aspartate aminotransferase; ALP, alkaline phosphatase; IL-Iβ, interleukin 1 beta; Caspase-3, cysteine-aspartic protease-3; Rat TNF-α, rat tumor necrosis factor-alpha; MDA, malondialdehyde; GSH, glutathione; Dox, doxorubicin; EB, erecta butanol; EE, erecta ethylactate. (* significant from -ve control &# significant from +ve control)