Fig. 2.

Redistribution of HNRNPA2B1 during EV-71 infection (A) Cytoplasmic protein (tubulin, TUB) and nucleoprotein (histone, HIS) were verified by WB analysis. (B) Un-infected and EV-71-infected cells were collected at 24 h post-infection time. HNRNPA2B1 protein expression was detected by WB analysis. (C) Image J analysis was performed to scan the protein abundance. The percentage of HNRNPA2B1 protein in the nucleus and cytoplasm in SK-N-SH cells was statistically analyzed. GAPDH was used as a control for qRT-PCR and WB analysis. (D) HNRNPA2B1 protein localization in SK-N-SH cells was detected by IF assay. Red signals, HNRNPA2B1. DAPI signals, nuclei. Scale bar = 5 μm. Data are from three independent experiments with each experiment performed in triplicate.