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. 2023 Sep 20;14:5849. doi: 10.1038/s41467-023-41506-0

Fig. 6. The effect of Polε docking to MCM ring on CMG helicase and leading strand DNA synthesis in vitro.

Fig. 6

a Schematic illustration of the unwinding of a 20-bp forked DNA by CMG. b, c Native PAGE of CMG helicase assays performed as in (a) in the absence (b) or presence (c) of Polε. d Quantification of the results; assays were performed in triplicate. Data are mean ± SEM (n = 3). Source data are provided as a source data file. e Scheme of reconstituting leading-strand DNA synthesis by Polε (see details in “Methods”). f Purified factors used for DNA replication assays analyzed by SDS-PAGE with Coomassie staining. g Alkaline agarose gel of reaction products from the replication assays performed as in (e). Similar results were obtained in two independent experiments.