Fig. 2. Transition from a pro-inflammatory to pro-remodeling macrophage is dysregulated in mast cell-deficient mice in a sex-specific manner.

a Representative contour plots for assessing macrophage polarization by CD206-PE versus CD86-BV786 labeling. Red values indicate the M2/M1 ratio per plot, and contour lines separate 5% of the events, each with outliers as individual black dots. Ratios of CD206⁺ M2 versus CD86⁺ M1 macrophage counts were assessed at 3, 7, and 11 days post-injection between wild-type (green), mast cell-deficient (red), and mast cell-engrafted (blue) male (b) and female mice (c). n = 4–9 per group. Box plots display a median with a 25–75% percentile distribution. d Median M2/M1 values between male (circles with solid lines) and female (squares with dashed lines) over time with statistical analysis between sexes at the same timepoint. Quantified results were pooled from 2–3 independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).