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. 2023 Sep 20;8:53. doi: 10.1038/s41536-023-00324-0

Fig. 4. Immune marker profiling and hormone receptor gene expression demonstrating differentiation expression based on mast cell presence.

Fig. 4

qRT-PCR gene expression in deficient and engrafted normalized to wild-type expression by 2−ΔΔct at day 11 in male mice for a Il1b and b Il33 relative to the housekeeping gene, Gapdh. c Gene expression ratio of receptor antagonist, Il1rn, relative to Il1b. d Gene expression of Cd206 relative to the housekeeping gene, Gapdh. qRT-PCR gene expression at day 3 in female mice measured for e Nos2, f Esr1, and g Esr2 relative to Gapdh. n = 11–16 per group for male samples, n = 6–8 per group for female samples. Data displayed as geometric mean ± geometric SD with results pooled from 2–3 independent experiments. (*p < 0.05, **p < 0.01, and ***p < 0.001).