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. 2023 Sep 20;13:15559. doi: 10.1038/s41598-023-42497-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Spectral data and bioactivity of fraction F4-1-2. (A) Base peak results of LC-MS/MS for fraction F4-1-2. (B) Mass range for 302.2185 m/z results of LC-MS/MS for fraction F4-1-2 and an EPA standard. (C) Mass spectral comparison between fraction F4-1-2 and EPA standard according to LC-MS/MS, observed for mass range of 302.2184 m/z. (D) Comparison of biological effect between fraction F4-1-2 and EPA standard after 48 h incubation on A549 cell line, compared to DMSO control. Data were obtained from 3 biological repeats ± SE and statistically analyzed with two-way ANOVA followed by a Tukey HSD means comparison test (***P < 0.001).