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. 2023 Sep 20;14:5832. doi: 10.1038/s41467-023-41391-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Bone marrow derived macrophages (A, B) and bone marrow neutrophils (C, D) from C57BL/6 and Nlrc4^-/- mice were primed 3 h with LPS and infected with PAO1, ∆pscD (no needle), ∆popB (no translocon), or ∆exoST. Quantification of IL-1β secretion by ELISA (A, C) and LDH release (B, D). E Caspase-1, GSDMD and IL-1β cleavage in infected macrophages and neutrophils from C57BL/6 and Nlrc4^-/- mice were examined by western blot. Macrophage westerns are combined whole cell lysate and TCA precipitated supernatants. Neutrophil whole cell lysates (WCL) and supernatants (SUP) were examined separately. Western blots are representative of 3 repeat experiments. Each data point in panels A-D represents one independent experiment (3-4 biological replicates). Statistical significance was assessed by 2-way ANOVA followed by Tukey’s post-test. Error bars are mean +/- SD.