Figure 4.

A guide for imaging BV2 cells using a confocal microscope at 63× magnification

(A) To decrease sampling bias, we recommend taking multiple images (≥ 9) from different areas of each well, such as in this grid example – red squares depict imaging acquisition areas. This is to ensure a representative capture of the whole well.

(B) Representative image of BV2 cells at 63× magnification with FUNCAT fluorescence in magenta.