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. 2023 Jul 10;4(3):102418. doi: 10.1016/j.xpro.2023.102418

Figure 7.

Figure 7

Expected FUNCAT fluorescence and quantification in BV2 microglia using microscopy

(A and B) (A) Composite images of FUNCAT fluorescence (magenta) and the nucleic marker DAPI (blue) in BV2 cells acquired using microscopy after 30 min (A) or 4 h (B) of AHA-labeling, with methionine and AHA with anisomycin (protein synthesis inhibitor) controls.

(C) Quantification of mean FUNCAT intensity per cell with 30 min AHA-labeling achieves sufficient FUNCAT signal from background controls, and this ratio is further increased by 4 h AHA-labeling (Data presented as mean ± SEM. One-way ANOVA with multiple comparisons, ∗∗∗∗p ≤ 0.0001, n > 70 cells per group).