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. 2023 Jul 10;4(3):102418. doi: 10.1016/j.xpro.2023.102418

Figure 8.

Figure 8

Expected quantification of FUNCAT signal in BV2 microglia using flow cytometry

(A and B) Following a 30 min AHA-labeling period, BV2 cells had significantly higher mean FUNCAT fluorescence intensity compared to methionine and AHA with anisomycin controls (one-way ANOVA with multiple comparisons, N = 3 with 30,000 cells per group, ∗∗∗∗p ≤ 0.0001).

(C) Following a 4 h labeling period, the number of BV2 cells above the methionine control threshold is further increased from 30 min labeling.

(D) Quantification of FUNCAT signal shows mean fluorescence intensity of FUNCAT positive AHA-labeled cells is significantly higher than methionine control (Student’s t-test, N = 3 with 9,000 cells per group, ∗∗∗p ≤ 0.001). Data presented as mean ± SEM.