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. 2023 Jul 10;4(3):102418. doi: 10.1016/j.xpro.2023.102418

Western blot transfer buffer

Reagent Final concentration Amount
Trans-Blot Turbo Buffer 5× 1× 200 mL
Methanol (100%) 20% (v/v) 200 mL
Milli-Q water N/A 600 mL
Total N/A 1 L

Store at 4°C for up to 3 months.