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Click chemistry reaction master mix
| Reagent | Amount per 100 μL |
|---|---|
| Component A: Reaction Buffer (prepared in before you begin (Prepare click chemistry reagents)) | 88 μL |
| Component C: Reaction Additive (prepared in before you begin (Prepare click chemistry reagents)) | 10 μL |
| 1 mM Alexa Fluor 647 alkyne (prepared in before you begin (Prepare click chemistry reagents)) | 0.2 μL (2 μM working conc.) |
| Component B: Copper (II) sulfate (CuSO4) | 2 μL |
| Total | 100 μL |
