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. 2023 Jul 12;4(3):102422. doi: 10.1016/j.xpro.2023.102422

Steinberg’s solution (Stock)

Reagent Final Concentration(mM) Amount(g)
KCl 6.7 0.5
Ca(NO3)2.4H20 3.4 0.8
MgSO4.7H20 8.3 2.0
NaCl 580 34.0
HEPES 499.37 119.0
Deionized (DI) Water N/A 1 L
Total N/A 1 L