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Schematic of the Pccac.398_401delAAGC allele
Exon 5 of the Pcca was targeted with a single-guide RNA (sgRNA) resulting in a 4-bp deletion after Cas9 cleavage and repair by non-homologous end joining (NEHJ). Shown are Sanger sequencing results of genomic DNA from a mouse with a WT Pcca genotype compared with a mouse homozygous for the Pccac.398_401delAAGC mutation (bottom). The figure was created with BioRender.
