Figure 6.

GUL-1 is involved in regulation of the CWI pathway. (A) Phosphorylation states of MAK-1 (45 kDa), as determined by probing with anti-phospho p44/42 antibodies, in the wild-type, Δgul-1, and Δmak-1 strains under standard growth conditions and 1 h following medium amendment with the chitin synthase inhibitor Nikkomycin Z 10⁻⁵ M (marked +). β-tubulin (50 kDa) was used as a reference protein. (B) Expression profiles of adv-1 in the wild-type and Δgul-1 strains as determined by RT PCR under standard growth conditions and 1 h following medium amendment with Nikkomycin Z (10⁻⁵ M). The relative expression level of the gene was analyzed by the 2^−ΔCt method with the β-tubulin gene as the internal control for normalization (n = 3), bars indicate ±1.96 SE (confidence interval of 95%). Asterisks indicate significant differences as determined by Student's t-text (p ≤ 0.05).