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. 2021 Sep 1;2:740845. doi: 10.3389/ffunb.2021.740845

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Copyright © 2021 Tisdale-Macioce, Green, Perl, Ashbaugh, Wiederhold, Patterson and Cushion.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The progression of lung organoid development. Each black arrow indicates an organoid. (A) Initial plating of primary lung epithelial cells (EpCAM) and primary lung fibroblast cells (CD140a) as viewed under light microscopy. (B) The development of lung organoids within 0.5–1.5 weeks. (C) The development of lung organoids between 1.5 and 2 weeks. (D) The development of lung organoids between 2 and 5 weeks of growth. All scale bars are 0.322 mm.