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Type 1 alveolar cell marker expression. Two independent organoids stained with Aquaporin 5 antibodies (AQP5); a membrane bound cell marker for type I alveolar epithelial cells pseudo labeled green. Left to right. (A) Bright field images organoids. (B) Nuclei stained with DAPI. (C) AQP5 (membrane bound staining pattern) of AEC1 cells. (D) Combination of nuclei and AQP5 staining. Scale bars are 50 μm.
