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. 2021 Sep 1;2:740845. doi: 10.3389/ffunb.2021.740845

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Copyright © 2021 Tisdale-Macioce, Green, Perl, Ashbaugh, Wiederhold, Patterson and Cushion.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Type 1 and Type 2 alveolar epithelial cell markers. Three independent organoids stained with both cell markers AQP5 (green) and SFTPC (red). From left to right. (A) Bright field images of organoids. (B) Stained with DAPI. (C) AQP5 5 staining. (D) SFTPC staining. (E) Merged image of all cell markers. Scale bars are 50 μm.