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. 2022 Oct 19;3:1029114. doi: 10.3389/ffunb.2022.1029114

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Copyright © 2022 Ingole, Nagarajan, Uhse, Giannini and Djamei

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of mCherry and eGFP with TetON system. (A) Growth of indicated U. maydis strains on Potato Dextrose agar (PDA)+CBX ± Tet (20 μg/ml). Plate photographs were taken on the 4th day after spotting. (B) Confocal images of cells from SG200:P _otef -mCherry-3xHA and SG200:P _otef -eGFP-3xHA after 4 days of growth on PDA plates (Scale bar= 20 μm). (C) Confocal images of cells from SG200:P _tet -mCherry-3xHA and SG200:P _tet -eGFP-3xHA after 4 days of growth on PDA+CBX ± Tet (20 μg/ml) plates (Scale bar= 20 μm). (D) Immunoblot with α-HA and α -Actin antibodies (loading control) on total protein extracts from indicated strains grown on PDA+CBX ± Tet (20 μg/ml) plates.