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. 2022 Oct 19;3:1029114. doi: 10.3389/ffunb.2022.1029114

Figure 4.

Figure 4

Dose-dependent induction of gene expression by TetON system. (A) The phenotype of fungal growth on the 4th and 7th day of indicated U. maydis strains grown on PDA+CBX ± Tet (2, 5, 10, 20, 50, and 100 μg/ml). (B, C) Immunoblot performed with α-HA, α-GFP, and α-Actin antibodies on total protein extracts of sporidia harvested from 4 days old, indicated strains.