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. Author manuscript; available in PMC: 2023 Sep 21.
Published in final edited form as: J Am Soc Mass Spectrom. 2022 Jun 1;33(7):1238–1249. doi: 10.1021/jasms.2c00072

Figure 1.

Figure 1.

(a) Positive ion mode nanoESI mass spectra showing the microdroplet digestion (top panel) and bulk digestion (bottom panel) of β-lactoglobulin B, inset: fully cleaved tryptic peptides from β-lactoglobulin B (peptides highlighted in red showing higher abundance in microdroplet); (b) a sequence coverage plot of β-Lactoglobulin B obtained from microdroplet digestion and bulk digestion based on fully cleaved peptides; Mass spectra of unmodified WENGECAQK in microdroplet (c) and deamidated WENGECAQK in bulk overnight digest (i.e., the deamidation product peptide WEDGECAQK) (d), respectively; NL indicates normalized level (NL) intensity.