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. 2023 Sep 21;21:247. doi: 10.1186/s12964-023-01099-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characteristics of nestin-expressing cells in the lung. A Flowcytometric analysis of nestin-expressing cells in a nestin-GFP murine lung and CD45⁻ lung cells. B The frequency of CD45⁻/nestin-expressing cells in 9–12-week-old male and female nestin-GFP murine lungs (n = 4–8 mice per group). C (left) Representative image of nestin-expressing cells in CD45⁻ lung cells obtained from a 2-week-old nestin-GFP mouse. Effect of aging on (middle) frequency of CD45⁻/nestin-expressing cells and (right) Nes (nestin) mRNA expression (n = 4–8 mice per group). D Representative images of cell surface markers expressed on CD45⁻/nestin-expressing cells. E Fluorescent microscopic images of a nestin-GFP murine lung. Nuclei are shown in purple-blue with DAPI. Scale bars = 50 μm. F GFP immunostaining of wildtype (WT) and nestin-GFP murine lungs. Nestin signals are shown in brown with anti-GFP antibody. Scale bars = 100 μm. G Expression of Nes mRNA between CD31⁺ endothelial cells and CD31⁻ non-endothelial cells obtained from murine lungs (n = 4 per group)