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. 2023 Sep 21;21:247. doi: 10.1186/s12964-023-01099-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Notch3 expression is regulated by nestin in vitro. A Hierarchical clustered heatmap showing the gene expression patterns (fold change < − 2.0 or ≥ 2.0) in nestin-expressing (Nes⁺) and -nonexpressing (Nes⁻) endothelial cells (EC, n = 2 per group). Each row and line represent one sample and differentially expressed genes, respectively. Red and blue indicate upregulation and downregulation, respectively. B Dot plot showing the absolute value of fold change of differentially expressed genes between Nes⁺ and Nes⁻ EC. Notch3 is presented at sixth place. C Pericyte markers (Notch3 and CD13) expressed on CD45⁻/nestin-expressing and -nonexpressing cells (n = 4 per group). D and E Effect of nestin (D, n = 8 per group) or Notch3 (E, n = 6 per group)-gene silencing in MS1 cells on mRNA expression. F Morphological changes in MS1 cells treated with control or Notch3 siRNA in 2D culture at 48 h. Scale bars = 100 μm