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. 2023 Sep 21;21:247. doi: 10.1186/s12964-023-01099-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Effect of nestin gene silencing in vivo. A (left) GFP immunostaining of nestin-GFP murine lungs administrated with control or nestin siRNA. Scale bars = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 9–13 per group). B Effect of in vivo nestin gene silencing on Notch3 expressing cells (n = 4 per group). C Effect of in vivo nestin gene silencing on mesenchymal-related mRNA expression in the lung (n = 6 per group). D Effect of in vivo nestin gene silencing on αSMA and Col1 expression in lung endothelial cells (n = 6 per group)