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. 2023 Sep 21;21:247. doi: 10.1186/s12964-023-01099-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Association between nestin-expressing cells and collagen production in bleomycin-induced pulmonary fibrosis. A Nes mRNA expression in murine lungs obtained at 2 d after administration with phosphate buffered saline (PBS, control group) or bleomycin (BLM, n = 4 per group). B Changes in CD45⁻/nestin-expressing cells during BLM-induced pulmonary fibrosis (n = 4–5 per group). C (left) GFP immunostaining of nestin-GFP murine lungs obtained at 14 d after administration with PBS or BLM. Scale bar = 100 μm. (right) Calculation of the GFP/nestin-positive area using Image J (n = 8 per group). D Flowcytometric analysis of the proportions of nestin-expressing cells in murine lungs 14 d after PBS or BLM treatment using anti-nestin antibody A and B. E The proportions of Notch3 and/or CD13-positive cells in the lungs obtained at 21 d after administration with PBS or BLM (n = 4 per group). F Correlations between the amount of hydroxyproline (indicating collagen content) and the number of CD45⁻/nestin-expressing cells in nestin-GFP murine lungs obtained at 7, 14, and 21 d after administration with BLM (n = 4–8 per group)