Figure 2.

Heatmap depicting color-coded Spearman’s correlation coefficients of circulating intestinal barrier function biomarkers and clinical outcome measures in the study participants. Correlation analysis of patients with FM (A), ME/CFS (B) and healthy controls (C) were evaluated using Spearman’s rank correlation test and FDR-adjusted P < 0.05. Pairwise Spearman’s rank correlation coefficients (rho) are depicted for each correlation and is presented by color intensity scale (at the top left of each panel). Heat color show standardized Z-scores (adjusted rho) across biomarkers and outcome measures. The color intensity is proportional to the strength of the association (rho value) ranging from red (positive correlations) to blue (negative correlations). Statistical significance was assessed using the Kruskal-Wallis test. FDR was calculated using Benjamini-Hochberg method. Statistical significance was set at *P < 0.05, **P < 0.01, and ***P < 0.001. Anti-β-LGB, anti-beta-lactoglobulin antibodies; ZO-1, zonulin-1; LPS, lipopolysaccharides; sCD14, soluble CD14; IL-1β, interleukin-1-beta.