Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 7;10:1261330. doi: 10.3389/fcvm.2023.1261330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Saito, Nose, Iida, Akazawa, Kanno, Fujimoto, Sasaki, Akehi, Higuchi, Akagi, Yoshida, Miyoshi, Ito and Nakamura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Generation of SLC5A5 transgenic human induced pluripotent stem cell (iPSC) lines. (A) Transduction of human SLC5A5 gene to AAVS1 locus in human induced pluripotent stem cells (iPSCs) using TALEN. (B) The difference in size of polymerase chain reaction (PCR) product depends on the presence or absence of the transgene. (C) Determination of the presence or absence of gene transfer to the AAVS1 locus using PCR of DNA extracted from each iPSC line. (D) EGFP expression in each human iPSC line. Scale bars are 200 µm.