Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 7;10:1261330. doi: 10.3389/fcvm.2023.1261330

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Saito, Nose, Iida, Akazawa, Kanno, Fujimoto, Sasaki, Akehi, Higuchi, Akagi, Yoshida, Miyoshi, Ito and Nakamura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

NIS-expressing human induced pluripotent stem cell (iPSC) lines showed slower proliferation in differentiation medium than NIS non-expressing human iPSC lines did. Sodium iodide (NaI) rescued poor proliferation of NIS-expressing cells. (A) NIS-expressing human iPSCs grew as well as NIS non-expressing human iPSCs in Essential 8 Flex Medium (maintenance medium for human pluripotent stem cells). Cells were cultured in Essential 8 Flex Medium for 4 days with or without NaI, and nuclei (blue) and F-actin (red) were stained. Scale bars are 1 mm. (B) Cell area/surface area was measured (N = 3 in each). *p < 0.001 (ANOVA/Bonferroni). (C) Cells were cultured with RPMI/B27 medium (basal medium for differentiation) for 4 days with or without NaI, and nuclei (blue) and F-actin (red) were stained. Scale bars are 1 mm. (D) Cell area/surface area was measured (N = 3 in each, Student-t test). (E) Cell counts were evaluated by MTT assay (N = 6 in each). *p < 0.01, **p < 0.001 vs. NIS (+) w/o NaI (ANOVA/Bonferroni).