Table 1.

PCR primers.

Name		Sequence	Product size (bp)	Annealing temperature (°C)
Subcloning PCR
Human SLC5A5	Forward	5′-TCATTTTGGCAAAGAATTCCACCGCCATGGAGGCCGTG-3′	1978	65
	Reverse	5′-ACGTAGCGGCCGCGATATCCTCAGAGGTTTGTCTCCTGCTGGTC-3′
Flanking PCR
Primer 1		5′-TGGGCTTTGCCACCCTATGCTGACA-3′		64
Primer 2		5′-ACAAGCAGAAGAACGGCATCAAGGTGAA-3′
Primer 3		5′-ACCAACCATCCCTGTTTTCCTAGGACTGA-3′