Table 1.
Primers used in this study.
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| 18S | 5’-AGAAACGGCTACCACATCCA-3’ | 5’-CCCTCCAATGGATCCTCGTT-3’ |
| MUC2 | 5’-GCCAGCTCATCAAGGACAG-3’ | 5’-GCAGGCATCGTAGTAGTGCTG-3’ |
| SPIB | 5’-GGCCACACTTCAGCTGTCT-3’ | 5’- CCCCTGTAGCCATCCATTCC-3’ |
| OCLN | 5’-ACAAGCGGTTTTATCCAGAGTC-3’ | 5’-GTCATCCACAGGCGAAGTTAAT-3’ |
| CLDN2 | 5’-ACCTGCTACCGCCACTCTGT-3’ | 5’-CTCCCTGGCCTGCATTATCTC-3’ |
| MTOR | 5’-ATGCTTGGAACCGGACCTG-3’ | 5’-TCTTGACTCATCTCTCGGAGTT-3’ |
| ATG5 | 5’-AAAGATGTGCTTCGAGATGTGT-3’ | 5’-CACTTTGTCAGTTACCAACGTCA-3’ |
| LYZ | 5’-CTTGTCCTCCTTTCTGTTACGG-3’ | 5’-CCCCTGTAGCCATCCATTCC-3’ |
| IAP | 5’-CTTTGGTGGCTACACCTTGC-3’ | 5’-CTCGCTCTCATTCACGTCTGG-3’ |
| LGR5 | 5’-CTCCCAGGTCTGGTGTGTTG-3’ | 5’-GAGGTCTAGGTAGGAGGTGAAG-3’ |
The 5’ to 3’ sequence of each primer pair (forward and reverse) are provided for each gene target.