Fig 3. Serum anti-rTsSPc IgG titers measured by ELISA.

The plate was coated using 1.5 μg/ml rTsSPc incubated at 4 °C overnight. After blockage with 5% skimmed milk and washes, the plate were probed with various dilutions of ten immune serum samples at 37 °C for 2 h. Normal murine sera (n = 30) diluted at 1:100 were determined as negative controls. HRP-conjugated IgG was used as the second antibodies. The coloration was developed by using the substrate OPD. The absorbance at 492 nm was measured after adding 2 M H₂SO₄. The cut-off value (0.201) is represented by a dotted line.