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. 2023 Sep 11;17(9):e0011629. doi: 10.1371/journal.pntd.0011629

Fig 5. Western blot analysis of TsSPc expression in different stages of T. spiralis.

Fig 5

A: SDS-PAGE of soluble crude proteins of diverse worm phases. Lane M: protein marker; Lane 1: ML; lane 2: IIL; lane 3: 3 d AW; lane 4: 6 d AW; lane 5: NBL; Lane 6: rTsSPc. B: Western blot analysis of native TsSPc in soluble somatic proteins of ML (Lane 1), IIL (Lane 2), 3 d AW (Lane 3), 6 d AW (Lane 4) and NBL (Lane 5) probed using anti-rTsSPc serum, and rTsSPc (lane 6) recognized by anti-rTsSPc serum was used as positive control. C: SDS-APGE analysis of ES antigens of different T. spiralis stages. Lane M: protein marker; lane 1–4: ES antigens of T. spiralis ML (lane 1), IIL (lane 2), 3 d AW (lane 3), 6 d AW (lane 4), and rTsSPc (lane 5). D: Western blotting of ES antigens of diverse T. spiralis phases. Native TsSPc in ES antigens of T. spiralis ML (Lane 1), IIL (Lane 2), 3 d AW (Lane 3), 6 d AW (Lane 4), and rTsSPc (Lane 5) were identified by anti-rTsSPc serum.