Table 1. TsSPc-specific dsRNA primers with T7 RNA polymerase promoter sequences*.
Primers | Sequences |
---|---|
T7-TsSPc-F1 | 5′-GATCACTAATACGACTCACTATAGGGAACCCAAATTACCCGGTGGG-3′ |
T7-TsSPc-R1 | 5′-GATCACTAATACGACTCACTATAGGGGCAATTTGTCATCCTTTTTCGGC-3′ |
T7-TsSPc-F2 | 5′-GATCACTAATACGACTCACTATAGGGTGGAAACGGTGCTGGAAGTT-3′ |
T7-TsSPc-R2 | 5′-GATCACTAATACGACTCACTATAGGGTGTCCACATCTACTTGTTTCAGTG-3′ |
T7-TsSPc-F3 | 5′-GATCACTAATACGACTCACTATAGGGGAAGCTTCTGCCCGAAAGGT-3′ |
T7-TsSPc-R3 | 5′-GATCACTAATACGACTCACTATAGGGAGCCAGGATGTTTACTATCACCA-3′ |
T7-GFP-F | 5′-GATCACTAATACGACTCACTATAGGGTCCTGGTCGAGCTGGACGG-3′ |
T7-GFP-R | 5′-GATCACTAATACGACTCACTATAGGGTTCTCGTTGGGGTCTTTG-3′ |
* The underlined sequence was the protective bases; the bold bases were the T7 promoter sequence.