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. 2023 Sep 11;17(9):e0011629. doi: 10.1371/journal.pntd.0011629

Table 1. TsSPc-specific dsRNA primers with T7 RNA polymerase promoter sequences*.

Primers Sequences
T7-TsSPc-F1 5′-GATCACTAATACGACTCACTATAGGGAACCCAAATTACCCGGTGGG-3′
T7-TsSPc-R1 5′-GATCACTAATACGACTCACTATAGGGGCAATTTGTCATCCTTTTTCGGC-3′
T7-TsSPc-F2 5′-GATCACTAATACGACTCACTATAGGGTGGAAACGGTGCTGGAAGTT-3′
T7-TsSPc-R2 5′-GATCACTAATACGACTCACTATAGGGTGTCCACATCTACTTGTTTCAGTG-3′
T7-TsSPc-F3 5′-GATCACTAATACGACTCACTATAGGGGAAGCTTCTGCCCGAAAGGT-3′
T7-TsSPc-R3 5′-GATCACTAATACGACTCACTATAGGGAGCCAGGATGTTTACTATCACCA-3′
T7-GFP-F 5′-GATCACTAATACGACTCACTATAGGGTCCTGGTCGAGCTGGACGG-3′
T7-GFP-R 5′-GATCACTAATACGACTCACTATAGGGTTCTCGTTGGGGTCTTTG-3′

* The underlined sequence was the protective bases; the bold bases were the T7 promoter sequence.