Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
UAS-nSynaptobrevin RNAi | Bloomington Drosophila Stock Center | BDSC 31983 | |
| Genetic reagent (D. melanogaster) |
UAS-dcr2 | Bloomington Drosophila Stock Center | BDSC 24650 | |
| Genetic reagent (D. melanogaster) |
UAS-Trh RNAi | Bloomington Drosophila Stock Center | BDSC 25842 | |
| Genetic reagent (D. melanogaster) |
UAS-ChAT RNAi | Bloomington Drosophila Stock Center | BDSC 25856 | |
| Genetic reagent (D. melanogaster) |
UAS-Tbh RNAi | Bloomington Drosophila Stock Center | BDSC 27667 | |
| Genetic reagent (D. melanogaster) |
UAS-Hdc RNAi | Bloomington Drosophila Stock Center | BDSC 26000 | |
| Genetic reagent (D. melanogaster) |
UAS-VMAT RNAi | Bloomington Drosophila Stock Center | BDSC 31257 | |
| Genetic reagent (D. melanogaster) |
UAS-GAD1 RNAi | Bloomington Drosophila Stock Center | BDSC 28079 | |
| Genetic reagent (D. melanogaster) |
UAS-DDC RNAi | Bloomington Drosophila Stock Center | BDSC 27030 | |
| Genetic reagent (D. melanogaster) |
UAS-DVGlut RNAi | Bloomington Drosophila Stock Center | BDSC 27538 | |
| Genetic reagent (D. melanogaster) |
UAS-sNPF RNAi | Bloomington Drosophila Stock Center | BDSC 25867 | |
| Genetic reagent (D. melanogaster) |
UAS-VGAT RNAi | Bloomington Drosophila Stock Center | BDSC 41958 | |
| Genetic reagent (D. melanogaster) |
UAS-TDC2 RNAi | Bloomington Drosophila Stock Center | BDSC 25871 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP1 RNAi | Bloomington Drosophila Stock Center | BDSC 32861 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP2 RNAi | Bloomington Drosophila Stock Center | BSC 32475 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP3 RNAi | Bloomington Drosophila Stock Center | BSC 31492 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP4 RNAi | Bloomington Drosophila Stock Center | BDSC 33682 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP5 RNAi | Bloomington Drosophila Stock Center | BDSC 31378 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP6 RNAi | Bloomington Drosophila Stock Center | BDSC 33684 | |
| Genetic reagent (D. melanogaster) |
UAS-dILP7 RNAi | Bloomington Drosophila Stock Center | BDSC 32862 | |
| Genetic reagent (D. melanogaster) |
UAS-amon RNAi | Bloomington Drosophila Stock Center | BDSC 29009 | |
| Genetic reagent (D. melanogaster) |
ISN-Gal4 (VT011155-Gal4) | FlyLight, Janelia Research Campus | Fly Light ID 54404 | |
| Genetic reagent (D. melanogaster) |
ISN-LexA (GMR34G02-LexA) | Bloomington Drosophila Stock Center | BDSC 54138 | |
| Genetic reagent (D. melanogaster) |
UAS-myrGFP.QUAS-mtdTomato-3xHA; trans-Tango | Bloomington Drosophila Stock Center | BDSC 77124 | |
| Genetic reagent (D. melanogaster) |
VT002073-Gal4.AD | Bloomington Drosophila Stock Center | BDSC 71871 | |
| Genetic reagent (D. melanogaster) |
VT040568-Gal4.DBD | Bloomington Drosophila Stock Center | BDSC 72902 | |
| Genetic reagent (D. melanogaster) |
UAS-csChrimson.mVenus | Bloomington Drosophila Stock Center | BDSC 55134 | |
| Genetic reagent (D. melanogaster) |
LexAop-ChrimsonR.mCherry | Gift from Jayaraman Lab | ||
| Genetic reagent (D. melanogaster) |
UAS-ArcLight | Bloomington Drosophila Stock Center | BDSC 51056 | |
| Genetic reagent (D. melanogaster) |
Empty split | Bloomington Drosophila Stock Center | BDSC 79603 | |
| Genetic reagent (D. melanogaster) |
ppk28-Gal4 | Cameron et al., 2010. | BDSC 93020 | |
| Genetic reagent (D. melanogaster) |
Gr5a-Gal4 | Chyb et al., 2003. | BDSC 57592, 57591 | |
| Genetic reagent (D. melanogaster) |
CCha2R-RA-Gal4 | Bloomington Drosophila Stock Center | BDSC 84603 | |
| Genetic reagent (D. melanogaster) |
LexAop-CsChrimson.tdTomato (III) | Bloomington Drosophila Stock Center | BDSC 82183 | |
| Genetic reagent (D. melanogaster) |
UAS-GCaMP6s (III) | Bloomington Drosophila Stock Center | BDSC 42749 | |
| Genetic reagent (D. melanogaster) |
20XUAS-GCaMP7b | Bloomington Drosophila Stock Center | BDSC 79029 | |
| Genetic reagent (D. melanogaster) |
CCAP-Gal4 (II) | Bloomington Drosophila Stock Center | BDSC 25685 | |
| Genetic reagent (D. melanogaster) |
CCAP-Gal4 (III) | Bloomington Drosophila Stock Center | BDSC 25686 | |
| Genetic reagent (D. melanogaster) |
CCHa2R (RA)-LexA | Bloomington Drosophila Stock Center | BDSC 84363 | |
| Genetic reagent (D. melanogaster) |
dILP2-LexA | Li and Gong, 2015. | ||
| Antibody | Anti-Brp (nc82) (Mouse monoclonal) |
DSHB, University of Iowa, USA | DSHB Cat# nc82, RRID:AB_2314866 | 1:40 |
| Antibody | Anti-GFP (Chicken polyclonal) |
Invitrogen | Thermo Fisher Scientific Cat# A10262, RRID:AB_2534023 | 1:1000 |
| Antibody | Anti-dsRed (Rabbit polyclonal) |
Takara, Living Colors | Takara Bio Cat# 632496, RRID:AB_10013483 | 1:1000 |
| Antibody | Anti-mouse AF647 (Goat polyclonal) |
Invitrogen | Thermo Fisher Scientific Cat# A-21236, RRID:AB_2535805 | 1:500 |
| Antibody | Anti-chicken AF488 (Goat polyclonal) |
Life Technologies | Thermo Fisher Scientific Cat# A-11039, RRID:AB_2534096 | 1:1000 |
| Antibody | Anti-rabbit AF568 (Goat polyclonal) |
Invitrogen | Thermo Fisher Scientific Cat# A-11036, RRID:AB_10563566 | 1:1000 |
| Chemical compound | All trans-Retinal | MilliporeSigma | Cat# R2500 | |
| Software, algorithm | Fiji | https://fiji.sc/ | RRID: SCR_002285 | |
| Software, algorithm | NAVis | Copyright 2018, Philipp Schlegel | ||
| Software, algorithm | CATMAID | Saalfeld et al., 2009; https://catmaid.org | ||
| Software, algorithm | Flywire | Flywire; https://flywire.ai/ | RRID:SCR_019205 | |
| Software, algorithm | GraphPad Prism | GraphPad Software; https://www.graphpad.com/scientific-software/prism/ | RRID:SCR_002798 | |
| Software, algorithm | Python | Python Software Foundation; https://www.python.org/downloads/ | ||
| Software, algorithm | Adobe Illustrator | Adobe Software; https://www.adobe.com/products/illustrator.html | ||
| Software, algorithm | CAVE (connectome annotation versioning engine) | https://github.com/seung-lab/CAVEclient/blob/master/FlyWireSynapseTutorial.ipynb; seung-lab, 2021; Buhmann et al., 2021; Eckstein et al., 2020; Heinrich et al., 2018 | ||
| Software, algorithm | R Project for Statistical Computing | Dessau and Pipper, 2008 | RRID:SCR_001905 |