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. 2023 Sep 7;14:1236906. doi: 10.3389/fmicb.2023.1236906

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Copyright © 2023 Lu, Shen, Wang and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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pHPLC analysis of the crude extract of strain TRM95458 fermented in liquid fermentation medium (Glycerol 2%, peptone 1%, glycine 1%, yeast extract 1%), 80% methanol eluent was purified by preparative HPLC, Conditions: A: 30% MeOH-H2O (0.075% HCOOH); B: 100% MeOH; flow rate:10mL/min; gradient elution conditions: 0–40 min 0–65% B; 40–45 min 65–100%B; 45–50 min 100%B; DAD detector wavelength: 350 nm. 2, tR = 19.38 min; 1, tR = 21.85 min; 3, tR = 28.28 min; and 4, tR = 33.13 min. The crude extract was isolated by pHPLC equipped.