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. 2023 Apr 28;25(11):3188–3202. doi: 10.1007/s12094-023-03187-5

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Anagliptin inhibits the differentiation and M2 polarization of mouse bone marrow-derived macrophages (BMDMs) in vitro. a, b, Flow cytometry analysis of the M0 marker (CD11b⁺F4/80⁺), M1 marker CD86, and M2 marker CD206 with anti-CD11b, anti-F4/80, anti-CD86, and anti-CD206 antibodies, respectively. ***, P < 0.01. c, Detection of M1 (nitric oxide synthase [iNOS], tumor necrosis factor-α [TNF-α]) and M2 (interleukin 10 [IL-10], arginase-1 [ARG-1]) markers by real-time polymerase chain reaction in the cells. ***, P < 0.01