TABLE 1.
Primers used for amplification of H. hepaticus urease genes
| Primerc | Sequence | Source of sequence (position) | Gene |
|---|---|---|---|
| HURE1 | GGAATTCTAGAATAGGAGAATGAGATGA | H. pylori (2634–2662)a | ureA |
| HURE3 | CCATCGATGCCCATATTATGGAAGAAGC | H. pylori (2777–2798)a | ureA |
| HURE4 | GGAGATCTCCACCAATCATGGTTGTTACA | H. pylori (3834–3855)a | ureB |
| HHUF1 | ATTAAATTTGGCGGAGGAAAA | H. hepaticus (817–837)b | ureB |
| HHUR1 | ATATGTCCCTAAATGTTTCGA | H. hepaticus (922–942)b | ureB |
| HHUF5 | TTGCATTATGCTGGGC | H. hepaticus (28–45)b | ureA |
| 4324R | GCATCCGCGGCCGCTGGTGGCACACCATAAGCATGTC | H. pylori (4324–4346)a | ureB |
a
Sequence from the published H. pylori urease sequence (29) at the position indicated.
b
Sequence from this study at the position indicated.
c
Primers HHUF5 and 4324R were used for RFLP analysis. Primers HHUF5 and HHUR1 were used to distinguish H. hepaticus from other Helicobacter spp.