|
Antibodies
|
|
| Chicken polyclonal anti-GFP antibody |
Aves Labs |
Cat#GFP-1020; RRID: AB_2307313 |
| Rabbit monoclonal (EP2730Y) to PKC (phospho T514) |
Abcam |
Cat#ab109539; RRID: AB_10863532 |
| Goat polyclonal anti-rabbit IgG H&L Alexa Fluor 488 |
Abcam |
Cat#ab150077; RRID: AB_2630356 |
| Mouse monoclonal anti- GAPDH |
Abcam |
Cat#ab8245; RRID: AB_2107448 |
| Donkey polyclonal anti-chicken IgY Alexa Fluor 488 |
Jackson ImmuneResearch |
Cat#703-545-155; RRID: AB_2340375 |
| Goat polyclonal anti-mouse IgG, HRP conjugated |
Santa Cruz Biotechnology |
Cat#sc2005; RRID: AB_631736 |
| Donkey polyclonal anti-rabbit IgG H&L, HRP conjugated |
Abcam |
Cat#ab205722; RRID: AB_2904602 |
|
|
Chemicals, peptides, and recombinant proteins
|
|
| Histamine |
Sigma-Aldrich |
Cat# H7125 |
| Chloroquine |
Sigma-Aldrich |
Cat# C6628 |
| Capsaicin |
Sigma-Aldrich |
Cat# M2028 |
| Tamoxifen |
Sigma-Aldrich |
Cat# T5648 |
| CNO |
Sigma-Aldrich |
Cat# C0832 |
| Naltrexone |
Sigma-Aldrich |
Cat# 1453504 |
| Ibuprofen |
Sigma-Aldrich |
Cat#I1892 |
| QX-314 |
Sigma-Aldrich |
Cat# 552233 |
| U73122 |
Sigma-Aldrich |
Cat# U6756 |
| Gallein |
Tocris Bioscience |
Cat# 3090/50 |
| DAPI |
Invitrogen |
Cat# E140588 |
| Optimal cutting temperature (OCT) compound |
SAKURA Tissue-Tek |
Cat# 4583 |
| Diphtheria toxin (DTX) |
Sigma-Aldrich |
Cat#D0564 |
| Resiniferatoxin (RTX) |
Tocris Bioscienc |
Cat#1137 |
| Dispase |
Gibco |
Cat# DN25 |
| Collagenase type I |
Gibco |
Cat#17018029 |
| poly-l-lysine |
Sigma-Aldrich |
Cat#P8920 |
| Nerve growth factor |
Sigma-Aldrich |
Cat#N8133 |
| Neurobasal medium |
Gibco |
Cat#21103049 |
| Fura 2 |
Invitrogen |
Cat#F1201 |
| RIPA lysis and extraction buffer |
Thermo Fisher Scientific |
Cat#89900 |
| protease inhibitor mix |
Thermo Fisher Scientific |
Cat#A32965 |
| RNase inhibitor |
Life Tech |
Cat#4458236 |
| TaqManTM Gene Expression Master Mix |
Life Tech |
Cat#4369016 |
| B-27 supplement |
Gibco |
Cat#17504044 |
| L-glutamine |
Gibco |
Cat#25030081 |
| Western HRP Substrate |
Millipore |
Cat# WBLUR0100 |
|
|
Critical commercial assays
|
|
| RNAscope Multiplex Fluorescent Reagent Kit V2 |
ACD |
Cat #323100 |
| Single Cell-to-CT TM Kit |
Thermo Fisher Scientific |
Cat #4458237 |
|
|
Experimental models: Organisms/strains
|
|
| Mouse: C57BL/6J |
The Jackson Laboratory |
JAX:000664 |
| Mouse: ThCreER
|
The Jackson Laboratory |
JAX:008532 |
| Mouse: MrgprDCreERT
|
The Jackson Laboratory |
JAX: 031286 |
| Mouse: B6N;129-Tg(CAG-CHRM3, mCitrine)1Ute/J(Gq-DREADD) |
The Jackson Laboratory |
JAX:026220 |
| Mouse: ROSA26iDrR
|
The Jackson Laboratory |
JAX:007900 |
| Mouse: B6;129S6-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9) |
The Jackson Laboratory |
JAX:007909 |
| Mouse: Piezo2flox/flox
|
The Jackson Laboratory |
JAX: 027720 |
| Mouse: Trpa1-KO |
The Jackson Laboratory |
JAX: 006401 |
| Mouse: PKCδflox/flox
|
RIKEN BRC |
Strain #: 06462 |
| Mouse: Trpv1-EGFP |
MMRRC |
033029-UCD |
| Mouse: Trpv1Cre
|
Mark Hoon, NIH |
N/A |
| Mouse: MrgprA3GFP-Cre
|
Xinzhong Dong, Johns Hopkins University, HHMI |
N/A |
|
|
Oligonucleotides
|
|
| RNAscope® Probe- MrgprA3 |
ACD |
Cat #548161 |
| RNAscope® Probe- Piezo2 |
ACD |
Cat #400191 |
| qPCR Taqman probes for Prkca |
Thermo Fisher Scientific |
Assay ID Mm00440858_m1 |
| qPCR Taqman probes for Prkcb |
Thermo Fisher Scientific |
Assay ID Mm00435749_m1 |
| qPCR Taqman probes for Prkcd |
Thermo Fisher Scientific |
Assay ID Mm00440891_m1 |
| qPCR Taqman probes for Prkce |
Thermo Fisher Scientific |
Assay ID Mm00440894_m1 |
| qPCR Taqman probes for Prkcg |
Thermo Fisher Scientific |
Assay ID Mm00440861_m1 |
| qPCR Taqman probes for Gapdh |
Thermo Fisher Scientific |
Assay ID Mm99999915_g1 |
|
|
Software and algorithms
|
|
| Prism 9 |
GraphPad |
https://www.graphpad.com/
|
| Adobe Photoshop CS6 |
Adobe |
https://www.adobe.com/
|
|
|
Other
|
|
| von Frey filament |
North Coast Medical |
Cat #NC12775 |
| Piezo Servo Controller |
Physik Instrument |
E-625 |
