Figure 3.

Effect of EV-iPSCs on gene and protein expression of RAS components. The mRNA expression of renin (A), angiotensinogen (B), and AT₁ receptor (C) was evaluated by RT-PCR. The protein expression of renin (D) and angiotensinogen (E) was evaluated by western blot. HPRT and β-actin were used as the reference gene and protein, respectively. The immunofluorescence of angiotensinogen and AT₁ receptor was evaluated and quantified by microscopy (F–G). Angiotensinogen and AT₁ receptor markers are observed in red, and the nucleus was stained with DAPI (blue). 40× magnification. Images are representative of independent experiments. The results are expressed as the mean ± SEM. p < 0.05: * versus group control; # versus group TGF-β.