Table 1.
Primer sequences used in RT-PCR.
| No | Gene | Primer sequence | Accession number | Size [bp] |
|---|---|---|---|---|
| 1 | BACE |
GAGTCCCTCACGCTGCAAAG TGGAGAGTGGGCAGGAGAAATC |
AF190726 | 199 |
| 2 | PSEN1 |
GTGTTCTACTTCGCCACGGA GCGATGGATGTTGGAAACCG |
NM_001362271 | 297 |
| 3 | Acetylcholinesterase |
GCATACACCTTCCCTGGCTT CTTGGGCTCTGGTGGCATAA |
X56518 | 229 |
| 4 | GSK-3β |
AGCTCTGATTGGCCACTGTC TGGGAAGGAGGGAGGAGATG |
NM_019827 | 936 |
| 5 | CDK5 |
TGAGGGTGTGCCAAGTTCAG GCATTAGCCAGCTCTCAGCA |
NR_156449 | 374 |
| 6 | ADAM10 |
CAAAAACACCAGCGTGCCAA ATGCTTCTCTGGATGTGCCC |
NM_007399 | 474 |
| 7 | NF-KB |
ACACATCCGGACCTCGCA TCTGAAGCTCTCTCCTCCGC |
NM_001319226.2 | 200 |
| 8 | MAPK/ERK |
ACTCACTTCACCAGGATGCG GCTGTCCTGTTGACCATCCA |
NM_005921.2 | 111 |
| 9 | Actin |
CGCCAGCCTCTGAAACTAGA ATAGATGGGCACGTTGTGGG |
NM_001272041 | 528 |
BACE, β-secretase; PSEN1, presenilin 1 (γ-secretase); GSK-3β, glycogen synthase kinase-3; CDK5, cyclin-dependent kinase 5; ADAM10, A disintegrin and metalloproteinase domain-containing protein 10 (α-secretase); bp, base pairs.