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. 2023 Sep 21;6:961. doi: 10.1038/s42003-023-05336-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The positions of the water molecules around subsite –1 of EcOpgG. A small purple sphere is shown as a tentative water molecule with poor electron density (Wat1). Wat2 and Wat3 and the other water molecules are shown in red and beige spheres, respectively. K413 and L417 are shown as cyan sticks. Chains A and B are shown in blue cartoon and green surface representations, respectively. The substrate is shown as a white stick. b Superposition showing the spatial position of Loop A in EcOpgG and EcOpgD. The superimposed Loop A in the EcOpgD complex is shown in purple. G440 and W441 are shown as lines. Wat1–3 molecules in EcOpgD are shown as light green spheres. EcOpgG is shown as a semi-translucent surface. L417 is shown in cyan. The water molecules of EcOpgG are shown as presented in (a), except that Wat1 (poor) is omitted. The Glc moieties at subsites –1 and –3 of EcOpgG are shown as white sticks. C5, C6, and O6 atoms of the Glc moiety at subsite –3 in EcOpgG are shown as spheres of van der Waals radii. The thick yellow arrow represents another proton network that leads to the solvent.