Table 2.
Specific activities of EcOpgD mutants.
| Specific activity (U/mg) | Relative activity (%) | |
|---|---|---|
| E209Q | 0.48 (0.028) | 3.5 |
| D300N | 0.13 (0.0048) | 0.90 |
| D351N | 3.3 (0.31) | 23 |
| E385Q | 0.28 (0.014) | 2.0 |
| D388N | 0.022 (0.0009) | 0.16 |
| E439Q | 0.77 (0.018) | 5.6 |
| E445Q | 1.9 (0.073) | 14 |
| R359A | 0.064 (0.0013) | 0.46 |
| T386L | 0.78 (0.024) | 5.6 |
| T386A | 0.80 (0.013) | 5.8 |
| T386S | 2.4 (0.059) | 17.6 |
| W441L | 0.87 (0.013) | 6.3 |
| W441F | 1.0 (0.013) | 7.3 |
| P78L | 8.2 (0.50) | 59 |
| P78A | 6.5 (0.064) | 47 |
| N82A | 12 (0.20) | 87 |
| Y356F | 2.2 (0.054) | 16 |
| Wild-type | 14 (0.33) | 100 |
Medians of triplicate experiments are presented.
Maximum absolute values between medians and the other data of the triplicate experiments are shown in parentheses.
Activity to produce 1 μmol of reaction product per minute is defined as 1 U (μmol/min).