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. 2023 Sep 21;14:5885. doi: 10.1038/s41467-023-41205-w

Fig. 2. STA of the floor region allows modelling of the BUNV fusion domain.

Fig. 2

STA was performed as in Fig. 1, however aligning the floor region trimer (as opposed to the tripod). Over 16,000 subtomograms were aligned resulting in a ~ 13 Å resolution average (GS-FSC). a, b Sections through the electron density of the floor region average atop the viral membrane (M). c, d Isosurface rendering of the electron density (c side view, d top view) of the floor region (gold) atop the viral membrane (grey). Translucent tripods of the head-stalk (blue-green) regions have been added for orientation. e, f Modelling of the LACV fusion domain (residues 949–1344; domain I red, domain II yellow, domain III blue) in C3 symmetry within the BUNV floor region, revealing the location of the fusion loops (fl; orange) and remaining density that could be occupied by Gn (Gn?; pink arrow) (post-fusion structure pdb: 7A57). The location of the head (light blue) and stalk (light green) domains are indicated in (e) for orientation. Scale bars: a, b = 5 nm; c, d = 2 nm.