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. 2023 Sep 21;6(12):e202301966. doi: 10.26508/lsa.202301966

Figure 3. MAP4K1 promotes the proliferation and inhibits the death of glioblastoma multiforme cells.

Figure 3.

(A) CCK8 assay of cellular viability at different times (24, 48, 72, and 96 h) in MAP4K1 knockdown (KD) or knockout (MAP4K1−/−) U87 and T98G cells and their control groups (NC, MAP4K1+/+), respectively (n = 15, the data were from three independent experiments). (B) 5-ethynyl-2-deoxyuridine assay of cell proliferation in MAP4K1-KD U87 and T98G or MAP4K1−/− T98G cells and their NC groups 48 h after seeding (n = 20, the data were from three independent experiments). Scale bar, 100 μm. (C) Flow cytometry analysis of the rates of dead cells, which indicates Annexin V- plus PI-positive cells 48 h after seeding (n = 3, the data were from an independent experiment, and the same experiment was repeated three times). (D) Cell cycle analysis by flow cytometry showed G2/M arrest in MAP4K1-KD or knockout glioblastoma multiforme cells 72 h after seeding (n = 3, the data were from an independent experiment, and the same experiment was repeated three times). Data are represented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (A, B, C, D) t tests (B, C) and two-way ANOVA (A, D).