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. 2023 Sep 2;26(10):107817. doi: 10.1016/j.isci.2023.107817

Table 1.

ERK2 steady-state kinetics vs. Sub-D or ATP

	WT ERK2 vs. Sub-D			WT ERK2 vs. ATP			ERK2(C159S) vs. Sub-D
	Untreated	2.5 μM H₂O₂	Change (p value	Untreated	2.5 μM H₂O₂	Change (p value	Untreated	2.5 μM H₂O₂	Change (p value
k_cat,app(s⁻¹)	8.4 ± 0.8	14.1 ± 1.5	1.68-f (0.02)	53.3 ± 6.6	55.7 ± 6.7	1.05-f (0.2)	8.4 ± 0.8	11.3 ± 2.6	1.35-f (0.4)
K_m,app (μM)	2.4 ± 0.3	5.8 ± 0.9	2.43-f (0.02)	19.9 ± 0.9	23.0 ± 2.6	1.15-f (0.2)	6.3 ± 1.8	5.9 ± 1.5	−1.07-f (0.5)
k_cat/K_m (M⁻¹s⁻¹)	3.8 x 10⁶	2.7 x 10⁶	−1.41-f (0.06)	2.7 x 10⁶	2.7 x 10⁶	−1.02-f(0.8)	1.9 x 10⁶	2.1 x 10⁶	1.13-f (0.7)