Table 3.
Whole genome sequencing results of the 16 induced mutants and their 8 parental isolates.
| Isolates | ST | gyrA | gyrB | parC | mexR | nfxB | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| nuc | prot | nuc | prot | nuc | prot | nuc | prot | nuc | prot | ||
| PSA-154 | 308 | ||||||||||
| PSA-154-IG cipro | 308 | 247 A > G 259 G > T | T83 > A D87 > Y | 260\u00B0C > G | S87W | ||||||
| PSA-154-IG levo | 308 | 259 G > T | D87 > Y | ||||||||
| PSA-021 | 664 | ||||||||||
| PSA-021-IG cipro | 664 | 1,397\u00B0C > T | S466 > F | 77 ins T | Frame shift | ||||||
| PSA-021-IG levo | 664 | 2,245\u00B0C > T | P749 > S | 271–272 ins C | Frame shift | ||||||
| PSA-115 | 3,137 | ||||||||||
| PSA-115-IG cipro | 3,137 | 1,395–1,404 del CTCCCAGGA | S466-E468 del | ||||||||
| PSA-115-IG levo | 3,137 | 1,425–1,436 del CCTGGGCTGTGG | L476 > I C478-G481 del | ||||||||
| PSA-242 | 810 | ||||||||||
| PSA-242-IG cipro | 810 | ||||||||||
| PSA-242-IG levo | 810 | 1,395–1,401 del CTCCCA 2108–2010 del TGG | S466-Q467 del L702 del E704 > Q | 271–282 del GACCAGCGCAGC | S88-R91del | deletion | |||||
| PSA-025 | 253 | ||||||||||
| PSA-025-IG cipro | 253 | 248\u00B0C > T | T83 > I | 260\u00B0C > T | S87L | 182–216 del TACTGAACCAGATCATCCAGGCCTGCGACCTGGAG | Frame shift | ||||
| PSA-025-IG levo | 253 | 1,459–1,460 ins ACA | N486 ins | 353-end del | E118-end del | ||||||
| PSA-026 | 244 | ||||||||||
| PSA-026-IG cipro | 244 | 2,245\u00B0C > T | P749 > S | 544 T > C | S185 > P | ||||||
| PSA-026-IG levo | 244 | 259 G > A | D87 > N | 374–388 del TCACCCCGGAGGAAC | Frame shift | ||||||
| PSA-285b | 244 | ||||||||||
| PSA-285b IG cipro | 244 | 259 G > T | D87 > Y | 537–547 del ACGGCGCTTCC | Frame shift | ||||||
| PSA-285b-IG levo | 244 | 1,334–1,335 ins TGT | C477 ins | 260–261 ins GCAAC | Frame shift | ||||||
| PSA-113a | 357 | ||||||||||
| PSA-113a-IG cipro | 357 | 248\u00B0C > T | T83 > I | 514–515 del TA | Frame shift | ||||||
| PSA-113a-IG levo | 357 | 247 A > G | T83 > A | 2,246\u00B0C > A | P749 > H | 235–244 del AAACCTGGTC | Frame shift | ||||
ST, sequence type; IG, induced against; nuc, nucleotide; prot: protein.