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. 2023 Sep 8;14:1156842. doi: 10.3389/fimmu.2023.1156842

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Copyright © 2023 Cui, Brady, Zhang, Anderson and Popova

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acute and chronic LPS-induced IL-17a+ CD3+ T cells are the major IL-17a expressing immune cells in the neonatal lung. Immature C57BL/6J mice were inoculated with LPS (L) or PBS (P) intranasally on DOL 3, 5, 7, and 10. Lungs were analyzed during acute (one day after 1 dose) and chronic (one day after 4 doses) exposure. Lung digests were subjected to flow cytometry. (A) Flow gating strategy for detection of IL-17a+ immune cells in the neonatal lung. After acute and chronic LPS (L) exposure there was a significant increase in the fraction (B) and total number (C) of IL-17a+CD3+T cells. (C) Acute LPS exposure also increased the per cell expression of IL-17a among the CD3+ T cells compared with PBS (P) controls. In contrast, LPS did not induce IL-17 expression in non-CD3 immune cells. *P<0.05, ns, non-significant (one-way ANOVA). Each open or black circle indicates one baby mouse. N=3-4 per group. One of at least two independent experiments is presented. **p<0.01, ***p<0.001, **** p<0.0001.