Figure 7.

NKG2D blockade attenuates acute and chronic LPS-induced pro-inflammatory chemokine and cytokine expression. Immature mice were inoculated with LPS intranasally and injected with IgG or anti-NKG2D antibody as described above. Lungs were harvested after acute and chronic LPS exposure. mRNA expression of CXCL1, CXCL2, CCL2, CCL3, IL-6, IL-1β, TNF-α, and GM-CSF was quantified by qPCR (N=3-4 neonatal mice per group, one of at two independent experiments is presented, mean±SEM, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, ns, non-significant, one-way ANOVA).